RESUMO
BACKGROUND: Malignant melanoma (MM) is a very aggressive tumour. Although surgical excision of MM in the early stages has a very good prognosis, it often fails to completely inhibit tumour progression. Methylene blue photodynamic therapy (MB-PDT) is a technique that induces tissue damage by reactive oxygen species (ROS). AIM: To investigate the efficacy of and potential use of MB-PDT in restraining the aggressiveness of MM by analysing levels of proliferating cell nuclear antigen (PCNA) and heparanase (HPSE, a molecular marker of cell invasion) in a mouse model. METHODS: Expression of PCNA and two HPSE isoforms were analysed using immunohistochemistry (IHC) after MB-PDT in mice. Tumour volume and weight were also measured. RESULTS: Two treatments with MB-PDT promoted a decrease of 99% decrease in tumour volume and 75% in tumour weight compared with untreated mice (P < 0.05). Using IHC, a decrease in expression of 75% for PCNA and 95% for both HPSE isoforms (P < 0.05) was found. CONCLUSION: MB-PDT is a cheap and efficient method of decreasing MM volume and thus disease progression. This reduction is mediated by downregulation of PCNA and heparanases.
Assuntos
Glucuronidase/metabolismo , Melanoma Experimental/tratamento farmacológico , Azul de Metileno/uso terapêutico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Antígeno Nuclear de Célula em Proliferação/metabolismo , Neoplasias Cutâneas/tratamento farmacológico , Animais , Biomarcadores Tumorais/metabolismo , Modelos Animais de Doenças , Inibidores Enzimáticos/uso terapêutico , Feminino , Imuno-Histoquímica , Melanoma Experimental/metabolismo , Melanoma Experimental/patologia , Camundongos , Camundongos Nus , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologiaRESUMO
Vitiligo repigmentation induced by cutaneous grafts from the gluteal region and stimulation with UV and trisoralen. The extent of repigmentation achived with this method was greater than the published data. We suppose that the stimulation with trisoralen over a cutaneous graft from a donor area not exposed to sunlight is more effective. This simple method is a good alternative for treatment of localized vitiligo.
Assuntos
Terapia PUVA , Transplante de Pele , Vitiligo/cirurgia , Adulto , Estética , Feminino , Humanos , Pigmentação da Pele , Retalhos Cirúrgicos , Vitiligo/tratamento farmacológicoRESUMO
Tres pacientes com epidermodisplasia verruciforme foram avaliados imunologicamente atraves de testes in vivo e in vitro. Segundo os parametros de avaliacao de imunidade celular por nos adotados, todos os tres pacientes mostraram-se imunodeprimidos. Um dos pacientes que apresentava carcinomas foi submetido a imunoestimulacao com dinitroclorobenzeno (DNCB) e posteriormente reavaliado
Assuntos
Adulto , Humanos , Masculino , Feminino , Verrugas , Imunidade CelularRESUMO
The authors report the clinical and histological aspects of a primary cutaneous anexial neoplasm with many local recurrences without metastatic dissemination. Some histopathologic and histogenic aspects were studied and compared with the bibliographical data.
